| Abstract No.: | A-B1045 |
| Country: | Canada |
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| Title: | LIVE IMAGING OF INNATE IMMUNE RESPONSE IN A NOVEL MOUSE MODEL REVEALS A ROLE OF TLR2 IN THE BRAIN INJURY IN VIVO |
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| Authors/Affiliations: | 1 Mélanie Lalancette-Hébert*; 1 Daniel Phaneuf; 1 Geneviève Soucy; 1 Yuan Cheng Weng; 1 Jasna Kriz;
1 Université Laval, Québec, QC, Canada
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| Content: | Microglia play a key role in the inflammatory response in the CNS. Activation of these cells is a major component of immune reaction after pathogen invasion or brain injury. After an immune challenge, a cascade of events occurs including the expression of various surface proteins as well as the production of pro-inflammatory cytokines and trophic factors. Considering that inflammation has a spatio-temporal component, we decided to generate a mouse model expressing the luciferase and the GFP genes under the transcriptional control of the toll-like receptor 2 (TLR2) promoter. Using biophotonic/bioluminescent imaging, we pursued the characterization of immune response following CNS trauma in two model such as cerebral ischemia and endotoxin (LPS) cerebral injection. Double immunofluorescence and in situ hybridization showed a different cell type expression pattern of TLR2 between the two different models of injury. TLR2 expression is observed in neurons and microglial cells after cerebral ischemia, however after LPS cerebral injection only microglial expression of TLR2 is found. Moreover, our real-time imaging results and 3D reconstruction of the TLR2 expression revealed a dynamic spatio-temporal activation profile in the brain of the transgenic mice after cerebral ischemia. Using biophotonic/bioluminescent imaging and transgenic mice models as a tool will help us to better understand real-time pathological changes associated with the brain inflammatory response and allow us to better select for therapeutic targets and disease markers.
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