| Abstract No.: | C-B3047 |
| Country: | Canada |
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| Title: | NOVEL CALCIUM BINDING PROTEIN, LCABP, MODULATES NEURITE OUTGROWTH IN CENTRAL NEURONS OF LYMNAEA STAGNALIS. |
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| Authors/Affiliations: | 1 Nasrin Nejatbakhsh*; 1 Kwokyin Hui; 1 Isabel Cardona; 1 Conghui Guo; 2 Ronald van Kesteren ; 1 Zhong-Ping Feng;
1 University of Toronto, ON, Canada; 2 Vrije Universiteit, Amsterdam, The Netherlands
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| Content: | Understanding the complex processes that regulate the various aspects of neurite behaviour is essential for unveiling the dynamics of synaptic plasticity, and hold great promise for future potential therapeutic strategies. Although the calcium ion dependence of neurite outgrowth initiation, elongation and branching has been demonstrated, the mechanisms that dictate these complex processes, including specific proteins involved, remain elusive. Various calcium binding proteins have been shown to regulate this process through a modulation of the intracellular calcium concentrations. Recently, a novel calcium binding protein (LCaBP) has been cloned from the neurons of Lymnaea Stagnalis (pond snail). The mRNA of this protein was found in neurite extensions of isolated neurons, indicating a potential role of this protein in local protein synthesis. We further used a double strand RNA interference approach with various dsRNA and siRNA of LCaBP to effectively knock down the expression of the LCaBP gene in Lymnaea Stagnalis neurons. We found that the neurite behaviour, including outgrowth, regeneration, elongation and branching, was markedly reduced after the treatment of LCaBP dsRNA/siRNA, but not affected by the control RNA treatment. Thus, this study indicates that LCaBP is involved in neurite outgrowth behaviour, possibly through a process of local protein synthesis.
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